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NRS 12, e001 | Figure 1

Gong P, Madak-Erdogan Z, Li J, Cheng J, Greenlief CM, Helferich WG, Katzenellenbogen JA and Katzenellenbogen BS (2014) Transcriptomic analysis identifies gene networks regulated by estrogen receptor α (ERα) and ERβ that control distinct effects of different botanical estrogens. Nucl Recept Signal 12, e001. doi:10.1621/nrs.12001


Figure 1. Experimental design and compounds studied (left panel) and comparison of E2 regulated genes obtained by RNA-Seq in this study vs. previous microarray analysis (right panel). Left panel: schematic of the experimental design for generating MCF-7 cells containing the three complements of ERα and ERβ, and for the cell treatments with BEs or E2, and preparation of the RNA-seq samples. Cells were treated with control vehicle (0.1% ethanol), genistein, S-equol or liquiritigenin (1 μM) or E2 (10 nM) for 24 h prior to harvest of RNA and further processing. Right panel: MCF-7 cells were exposed to 10-8 M estradiol (E2) for 24h and RNA was isolated and analyzed by RNASeq (this study) or previously by Affymetrix microarrays (ref. 14). Venn diagram shows the number of genes regulated by E2 and the percent of E2-regulated genes found to be regulated by microarray analysis that overlap with those found by RNA-seq analysis.