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NRS 12, e003 | Figure 2

Filgueira CS, Benod C, Lou X, Gunamalai PSVillagomez RA, Strom A, Gustafsson , Berkenstam AL and Webb P (2014) A screening cascade to identify ERβ ligands (2014)  Nucl Recept Signal 12, e003. doi:10.1621/nrs.12003


Figure 2. DSF assay to determine the binding of ligands from compound libraries to ERβ LBD. Representative DSF output from the RT-PCR machine as graphs displaying the first derivative of fluorescence versus temperature for (A) unliganded ERβ LBD and unliganded ERβ LBD with DMSO and (B-D) ERβ LBD with the addition of ligand (final concentration 500 μM). Ethinyl estradiol (EE) is represented by the purple trace in B.