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NRS 12, e003 | Figure 5

Filgueira CS, Benod C, Lou X, Gunamalai PSVillagomez RA, Strom A, Gustafsson , Berkenstam AL and Webb P (2014) A screening cascade to identify ERβ ligands (2014)  Nucl Recept Signal 12, e003. doi:10.1621/nrs.12003

 



Figure 5. Cell-based transactivation assay to evaluate the ability of ligands to modulate the transcriptional activity of ERβ. (A) Relative transactivation activity for selected ligands (final concentration 1 μM) using HeLa cells transiently transfected with full length ERα and full length ERβ. (B) Cell viability assay measured in LNCaP cells after 24 h treatment with selected ligands at a final concentration 1 μM. Average of three wells each for three independent experiments.