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NRS 12, e004 | Figure 1

Liu X and Giguère  (2014) Inactivation of RARβ inhibits Wnt1-induced mammary tumorigenesis by suppressing epithelial-mesenchymal transition. Nucl Recept Signal 12, e002. doi:10.1621/nrs.12003

 

Figure 1. Ablation of Rarb suppresses Wnt1-induced mammary tumorigenesis and affects the composition of the stroma. (a) Rarb-null mice show a significant delay in tumor formation when the expression of Wnt1 is driven by the MMTV-LTR promoter (n=25 Rarb-/- and 26 wild-type mice). Insets: Western blot analysis of RARβ and Wnt1 expression in wild-type and Rarb-/- mice; immunostaining of RARβ in tumor (T) and stroma (S) in wild-type tissue. (b) Tumor growth rate as measured as time to end point (tumor burden total of 2 cm3), n=24. (c) Cell proliferation rate was identified by Ki67 expression (red signal), which is significantly lower in the tumors from Rarb null mice. (d) TUNEL analysis showed significantly increased apoptotic cells in Rarb null mice. (e) Caspase 3 is detected in tumor cells with a significant increase in Rarb null animals. (f) Mammary tumor paraffin sections stained for collagen 1 (Col3a1) show a decrease in matrix stiffness. Quantification of reactive stroma index (RSI) is shown on the right panel. (g) Growth rate of CAFs generated from control and Rarb-null mammary glands in vitro.. For panels c, d and e, scale bars, 50 and 20 μm for low (left) and high magnification (right), respectively. For panel f, scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P <0.001