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NRS 12, e004 | Figure 2

Liu X and Giguère  (2014) Inactivation of RARβ inhibits Wnt1-induced mammary tumorigenesis by suppressing epithelial-mesenchymal transition. Nucl Recept Signal 12, e002. doi:10.1621/nrs.12003

 

Figure 2. Loss of Rarb alters the gene expression profile of the stromal compartment and its composition. a) Differential gene expression analysis of the microdissected stromal compartment of MMTV-Wnt1 and MMTV-Wnt1/Rarb-/- tumors. Data are presented as relative fold change (log2) vs. wild-type stroma. (b) Mammary tumor paraffin sections stained for collagen 1 (COL3A1) show a decrease in matrix stiffness and indicate reduced activity of the CAFs in the Rarb-null tumor microenvironment. Quantification of positive area is shown on the right panel. (c) Paraffin sections stained for α-SMA (red, cytoplasmic). Quantification of positive area is shown on the right. (d) Mammary tumor paraffin sections stained for CD45 demonstrate a decreased infiltration of inflammatory cells in the Rarb-null stroma. Quantification of positive cells is shown on the right panel. (e) Mammary tumor paraffin sections stained for CD31 demonstrate a reduction of angiogenesis in the Rarb-null tumor microenvironment. Quantification of positive area is shown on the right panel. For all panels, scale bars, 50 and 20 μm for low (left) and high magnification (right), respectively.