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NRS 13, e004 | Figure 1

Puhl A, Milton F, Cvoro A, Sieglaff D, Campos J, Bernardes A, Filguiera C, Lindemann JL, Deng T, Neves F, Polikarpov I and Webb P (2015) Mechanisms of peroxisome proliferator activated receptor γ regulation by non-steroidal antiinflammatory drugs. Nucl Recept Sig 13, e004. doi:10.1621/nrs.13004


Figure 1. NSAIDs bind PPARγ and modulate PPARγ activity. (A) Results of displacement assay using fluorescently labeled ligand. Y-axis represents fluorescence polarization units, X-axis represents concentration of the PPARγ competitor ligands rosiglitazone, sulindac, diclofenac, indomethacin, and ibuprofen. Error bars represent one standard deviation from the mean of triplicate reaction wells. (B) Results of HeLa cell transactivation assays. Transfections used a PPARγ expression vector and a PPAR response element driven luciferase promoter. Cells were treated with varying concentrations (10-9 to 10-3 M) of rosiglitazone, sodium diclofenac, indomethacin and ibuprofen. Assays were performed in three replicates and normalized for differences in efficiency by measuring internal control Renilla luciferase activity in the same lysate. Error bars represent standard deviation.